R software (version 3.6.3) and GraphPad Prism v7.00 (GraphPad Software Inc.) were used as statistical analysis tools in this study. Quantitative data are presented as the mean ± standard error of the mean (SEM) or standard deviation (SD). The Wilcoxon test was applied to compare the statistical differences between the two groups, and the Kruskal‐Wallis H test was employed to compare multiple groups. Statistical significance was defined as P < .05. Venn diagrams were drawn using jvenn. 33 The other plots were constructed using R software or GraphPad Prism.

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