All samples were treated in a RIPA lysis buffer to collect total protein, which was then separated by 10% SDS‐PAGE gel and transferred onto a nitrocellulose membrane. After being probed sequentially with primary anti‐EYA3 antibodies (1:5000, ab95876, Abcam, Cambridge, MA) and HRP‐conjugated secondary antibodies (1:5000, ab6789, Abcam, Cambridge, MA), the protein blots were visualized by using a chemiluminescence reagent to quantify EYA3 expression with ImageJ v1.4.9 software.

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