The HTR8/SVneo cell migration ability was assessed by scratch‐wound and transwell assays as described previously. Briefly, HTR8/SVneo cells were transduced with either the shNT or the shINO80 lentivirus. Three days after infection, cells were either subjected to a scratch with a pipette tip or were preplated in transwell chambers, and images were taken at indicated time‐points. The distance of the wound edge or cell number across the transwell chambers was measured by ZEN analysis software at 12 hours.

The Matrigel cell invasion assay was carried out as previously described. We used Invasion Chambers (354480, Corning), which were pre‐coated with extracellular matrix proteins. The next steps were as described above. The cell number of cells that moved across the transwell chambers was calculated by ZEN analysis software at 24 hours.

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