For lipid droplet staining, different groups of SW480 and HCT116 cells incubated with 400 μM OA for 6 hours, or 10 μm cryostat sections from indicated CRC xenografts, were washed, fixed in 4% paraformaldehyde for 10 minutes and rinsed with 60% isopropanol. The slides were then placed in the freshly prepared working Oil Red O solution for 10 minutes at room temperature and rinsed again with 60% isopropanol. After lightly stained nuclei with haematoxylin and washed with distilled water, the slides were covered with glycerine jelly that will harden after a few hours. Relative lipid content was quantified by using Image Pro Plus 6.0.

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