CRC cell staining with Annexin V and PI was carried out using an Annexin V‐FITC/PI Apoptosis Detection kit (Merck). A total of 1 × 106 cells were incubated at 37°C for 30 minutes before centrifugation to collect the cell pellet, then resuspended in a Ca2+‐enriched binding buffer and analysed using a Beckman Coulter flow cytometry. Data were calculated using Cell Quest software.

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