Hippocampal senescence was detected using a β‐galactosidase staining kit. The working liquid was prepared as follows: staining fluid C 930 µL, staining fluid A 10 µL, staining fluid B 10 µL and X‐Gal fluid 50 µL were sequentially added and mixed. Fresh frozen sections were air dried, fixed with the β‐galactosidase fixative solution for 15 minutes and washed three times with distilled water. To stain the sections, several drops of working liquid were placed upon each section and incubated at 37°C for 16‐18 hours, before being washed three times with distilled water and coverslipped with glycerine jelly.

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