After fixed in 4% paraformaldehyde, cells were blocked with goat serum at 37°C for 1hour. They were incubated with rabbit Ki67(Millipore, AB9260) antibodies at 4°C overnight, then were incubated with FITC conjugated goat anti‐rabbit IgG (Dako, K500711) at 37°C for 1 hours after three times washing. Finally, the cell nucleus was stained with DAPI (Sigma‐Aldrich, D9542).

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.