After fixed in 4% paraformaldehyde, cells were blocked with goat serum at 37°C for 1hour. They were incubated with rabbit Ki67(Millipore, AB9260) antibodies at 4°C overnight, then were incubated with FITC conjugated goat anti‐rabbit IgG (Dako, K500711) at 37°C for 1 hours after three times washing. Finally, the cell nucleus was stained with DAPI (Sigma‐Aldrich, D9542).

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