2.6. Antibody staining and flow cytometry
This protocol is extracted from research article:
Novel chimeric TLR2/NOD2 agonist CL429 exhibited significant radioprotective effects in mice
J Cell Mol Med, Feb 19, 2021; DOI: 10.1111/jcmm.16252

The cell apoptosis was analysed using the apoptosis detection kit. After radiation, bone marrow cells (BMCs) were isolated freshly. Then, cells were strained through a 40‐μm strainer in the presence of PBS and red blood cells were removed. Cells were stained with antibody for 20 minutes at 4°C. TenF thousand cells were analysed by flow cytometry (Beckman Cytoflex) in accordance with the manufacturer's instructions.

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