Paraffin‐embedded tissue sections were de‐waxed as well as rehydrated before they were subject to antigen retrieval. Then, the sections were blocked with BSA, incubated with primary and secondary anti‐CX43 antibodies (Abcam), developed by using a VECTASTAIN Elite ABC assay kit (Vector Laboratories) according to the experimental protocol provided by the manufacturer and counter stained with DAPI before the expression of CX43 was evaluated.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.