Paraffin‐embedded tissue sections were de‐waxed as well as rehydrated before they were subject to antigen retrieval. Then, the sections were blocked with BSA, incubated with primary and secondary anti‐CX43 antibodies (Abcam), developed by using a VECTASTAIN Elite ABC assay kit (Vector Laboratories) according to the experimental protocol provided by the manufacturer and counter stained with DAPI before the expression of CX43 was evaluated.

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