2.9. Alkaline phosphatase (ALP) staining and activity

After the designated treatment, the cells were fixed for 3 minutes and stained with ALP (Nanjing Jiancheng Bio‐Engineering Institute Co. Ltd.) for 15 minutes according to the manufacturer's instructions. Besides, ALP activity and protein concentrations were detected with ALP activity kit and BCA assay kit, respectively. ALP activity was evaluated with normalization to the protein.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.