For the transwell assay, after the transfection of miR‐130a mimics, siRNA‐ATG2B or pCDNA3.1(+)‐ATG2B, VSMCs were resuspended in serum‐free DMEM (5 × 105 cells/mL), and 200 µL of the solution was added to the upper transwell chamber (Costar, NY). The lower chamber was filled with DMEM (Gibco) supplemented with 10% FBS (Gibco). After 24 hours of incubation, the cells that migrated to the lower surface of the chamber membrane were fixed with paraformaldehyde (4%) and subsequently stained with 0.1% crystal violet in 20% methanol, whereas the cells that remained on the upper surface of the membrane were removed with a cotton swab. After 3 washes with PBS, the migrated VSMCs were counted under an inverted microscope (Leica DMI4000B).

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