We described the basic demographics of SARI cases and the frequency of influenza A‐ and influenza B–positive results among SARI cases. Differences in characteristics between groups were assessed using chi‐square, with P‐values <.05 considered statistically significant. We also assessed sensitivity and specificity of the FTD‐33 Kit for detecting influenza A, influenza B, and the influenza A(H1N1)pdm09 strain by comparing results of the FTD‐33 Kit to those of the CDC rRT‐PCR panel, considering the CDC rRT‐PCR panel as the gold standard. Other influenza subtypes are unavailable for comparison in the FTD‐33 Kit, and influenza C was not tested by CDC rRT‐PCR panel. We also assessed sensitivity and specificity, limiting the cases to those that were collected during months in which there were greater than three specimens positive for influenza A, influenza B, or influenza A A(H1N1)pdm09 by either laboratory method. For influenza A, influenza B, and the H1N1 assays, we assessed cycle threshold (ct) values of discordant and concordant results. Analysis of concordant positive results relied on the reported FTD‐33 ct value, and analysis of the discordant results relied on the ct value of the positive assay. We conducted chi‐square tests to compare the proportion of concordant and discordant results with ct values above and below 30 for the influenza A, influenza B, and H1N1 assays. P‐values <.05 were considered statistically significant. Statistical analyses were conducted using SAS version 9.4 (SAS Institute Inc).

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.