The expression level of FSCN1 in each sample was determined through Western blotting. In brief, the samples were lysed in a radioimmunoprecipitation buffer to extract total protein, which was then resolved on 10% SDS‐PAGE. In the next step, the resolved proteins were electrophoretically blotted onto polyvinylidene fluoride (PVDF) membranes, which were then blocked with 5% skim milk at ambient temperature for 1 hour. Then, the membranes were incubated along with anti‐FSCN1 (1:1000, Abcam) primary antibodies and HRP‐labelled secondary antibodies under conditions suggested by the antibody manufacturer. Finally, the membranes were developed in an enhanced chemiluminescence (ECL) reagent (Thermo Fisher Scientific) and the densitometry analysis of protein bands was performed using ImageJ software to determine the relative protein expression of FSCN1 in each sample.

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