A DNA extraction kit (Huashun, Shanghai, China) was used to isolate the genomic DNA from VSMC according to the instructions of the manufacturer. A TaqMan genotyping kit (Applied Biosystems) was used to perform the genotyping of rs884225, rs17337023, rs325797437, rs344501106, rs81286029 and rs318656749 SNPs in collected clinical samples. Primer Express® Software v.3.0 (Applied Biosystems) was used to determine the genotype of these SNPs. A fluorescein dye with a minor groove binder (MGB) was used to label the 5’ end of probes specific for each allele of the SNPs. A 7900 Fast Real‐Time PCR System (Applied Biosystems) was used to perform the PCR using 96‐well plates. Positive and negative controls were included in each PCR plate to ensure the accuracy of the genotyping. Each test was repeated at least three times.

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