Hearts were fixed in 4% paraformaldehyde solution, embedded in paraffin and sectioned (5 μm thickness). After dehydration, sections were stained with haematoxylin and eosin (H&E) and then viewed under a microscope (Olympus, Tokyo, Japan). For quantification, cell area measurements were performed on five similar sections, and 100 nucleated cells were randomly selected to measure the mean cell area.

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