TRIzol (Invitrogen) was used for total RNA isolation. Total DNAs were synthesized by conducting the PrimeScript RT reagent Kit (Takara Bio). Real‐time PCR was performed by using Real Master Mix (SYBR Green). RNase R (Epicentre) was used to digest RNAs, remove the liner RNAs and enrich the circular configuration. The primers used in this study are as follows: circCAMSAP1 forward: GTGTCAAGCGCTTCTCAACG, reverse: GCTGGACAGGAGAAGCTTGA; miR‐1294 forward: TGTGAGGTTGGCATTGTTGTCTGT, reverse: GTGCAGGGTCCGAGGTATTC; GRAMD1A forward: ACACAATGGGCTACTGTGAGG, reverse: GGCTTGGTCTCGATGCTACT; and GAPDH forward: CGCTCTCTGCTCCTCCTGTTC, reverse: ATCCGTTGACTCCGACCTTCAC. 2−ΔΔCt method was used to calculate the relative expression levels of miR‐1294 and GRAMD1. Experiment was repeated three times.

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