The fresh tumor specimens were obtained after the surgery then washed three times using PBS. Thereafter, tumor tissues were cut using scissors and added with Trizol (TaKaRa, Tokyo, Japan). To detect mRNA expression in tumor cell lines, tumor cells were transfected with siTYRP1 for 48 h then cells were obtained. The concentration and purity of total RNA were detected by NanoDrop 2000 (Thermo Fisher Scientific, MA, USA). Exactly 1 ug of total RNA was used to reverse into cDNA using ReverTra Ace qPCR RT Kit (TOYOBO, OSAKA, Japan). The primers used were designed and purchased from Sangon Biotech (Shanghai, China) ( Supplementary Table 1 ). Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as control.

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