The siRNA sequence was designed by BIODEV ( and synthesized by Sangon Biotech (Shanghai, China). The sequence of si 1F: 5′-GGUCUUAACUACUAUGUUAUA-3′, R:5′-UAACAUAGUAGUUAAGACCAG-3′andsi2F:5′-GGUUCUGAUUAUUACGUUAAU-3′, R:5′-UAACGUAAUAAUCAGAACCUG-3′. Sangon Negative Control siRNA was used as control. The cells were seeded in an antibiotic-free complete medium at a density of 5 × 105 cells and cultured for 24 h. Transient transfection of cells with siRNA for 24 h was performed using Lipofectamine 2000 based on the manufacturer’s protocol. At 24 h after transfection, the medium was changed and the cells were allowed to recover.

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