SGE-301 was a gift from Sage Therapeutics (Cambridge, MA) (Paul et al., 2013). Other chemicals were purchased from Sigma Chemical Company (St. Louis, MO). For ex vivo slice experiments, SGE-301 or 24S-HC was dissolved in DMSO and administered at concentrations noted in the text. The concentration of DMSO was typically 0.1% and has no effect on hippocampal responses or synaptic plasticity (Izumi and Zorumski, 2020; Popiolek et al., 2020). For in vivo experiments, 5 mg SGE-301 was dissolved in 3.3 ml of 2-hydroxypropyl-β-cyclodextrin (CDX) (catalog number 332607; Sigma) (1.5 g CDX + 3 ml water) and administered at a final dose of 3 mg/kg i.p. This dose of SGE-301 was selected based on prior behavioral studies we have done with this agent (Paul et al., 2013).

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