Larval skeletal samples (phf21aa and kdm1a) were prepared and stained using Alcian Blue and Alizarin Red dyes as described (Walker & Kimmel, 2007; DeLaurier, Alvarez & Wiggins, 2019). Samples were flat-mounted and imaged using an Olympus BX41 compound microscope and Olympus cellSens Standard software (version 1.16).

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