HepG2 cells were exposed to cisplatin (DDP, Sigma-Aldrich, Germany) at the concentration of 0.1 mM, and followed by a sub-culture in the RPMI-1640 medium containing stepwise-increasing concentrations of DDP (0.1, 0.5, 1.0, 2.0 and 3.0 mM), timely. Finally, viable HepG2 cells in the culture medium with a high concentration of DDP (2.0 mM) were selected and defined as cisplatin-resistant HepG2 cells (HepG2/DDP).

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