The distribution of conserved sequences among different introns was assessed by the Mann–Whitney U test. The frequency distribution of the four groups (TFBS, TFBS-SS, SS, and not identified) among introns was analyzed using the Chi-square test. The number of TFBS as well as SS in conserved and non-conserved sequences was analyzed by the Wilcoxon paired non-parametric test. The number of TFBS for each TFBD family, between conserved and non-conserved patterns, was assessed by the Chi-square test. All the statistical analyses were performed in GraphPad Prism version 7.04 (GraphPad Software, La Jolla, California, USA). Statistically significant differences were set to ns: Not significant, *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001.

Ethical approval: The conducted research is not related to either human or animal use.

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