The transfected Caki-1 and 786-O cells were plated in 6-well plates. Later, a linear wound was created by scraping the cells with a sterilized pipette tip. After 24 h of incubation, the migration distance was photographed using a microscope at 100× magnification and calculated using ImageJ 1.8.0 software (National Institutes of Health, Bethesda, MD, USA).

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