After transfection, Caki-1 and 786-O cells were trypsinized and then seeded into 6-well plates. The culture medium was changed every 3 days for 2 weeks. Subsequently, the cells were fixed with formaldehyde and stained with crystal violet (Solarbio). Finally, the number of colonies was counted in five randomly selected fields under a microscope.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.