For immunofluorescence (IF), the cells on 24 × 24 mm glass slides were fixed with paraformaldehyde (4%, RT, 30 min), and then permeabilized with Triton X − 100 (0.5%, RT, 20 min). After washing, the cells were blocked with bovine serum albumin (5%, RT, 1 h), and then incubated with primary antibodies (Additional file 1: Table S2, 4 °C, 24 h). After washing with Tween20 (0.1%, 3 times, 15 min), cells were incubated with secondary antibodies (away from light, RT, 1 h). The images were taken by fluorescent or confocal microscope. For the xenograft tumor tissues, IF, immunohistochemistry (IHC) and hematoxylin and eosin (H&E) staining was performed by Biofavor Biotech, China.

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