The Pierce Magnetic RNA Protein pull-down Kit (Thermo Fisher Scientific) was performed to detect the interaction between circATP5B and SRSF1 according to the manufacturer’s instructions. Firstly, purified RNA was labeled by the biotinylated RNA probes, and then the positive control (input), negative control (antisense RNA), and biotinylated RNA were mixed and co-incubated with the proteins of GSCs at room temperature. The RNA-protein complex was added with magnetic beads to prepare a probe-magnetic bead complex. Finally, the complexes were detected by western blotting after being washed and boiled, and β-actin was used for the control.

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