Luciferase reporter assays were performed as described previously [26]. Firstly, the luciferase reporter plasmids (circATP5B-wt and circATP5B-mt, HOXB5–3′-UTR-wt and HOXB5–3′-UTR-mt, IL6-wt and IL6-mt, and SRSF1-wt and SRSF1-mt) were constructed by Gene-Chem (Shanghai, China). The GSCs were then seeded into 96-well plates at a density of 5 × 103 cells/well, transfected with luciferase reporter plasmids and performed other relative treatment for 48 h. Finally, the relative luciferase activities were detected via a Dual-Luciferase Reporter Assay System (Promega, USA). Relative luciferase activity was calculated as the ratio of firefly luciferase activity to Renilla luciferase activity. All experiments were independently repeated in triplicate.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.