Before AcSD sessions, CD-1 mice were primed for aggressiveness in two phases. In the first phase, an adult (PND65) male C57BL/6 mouse was introduced to a CD-1 mouse’s home cage for 3 min or until it was attacked 10 times, whichever came first, on three consecutive days. On the fourth day, the second phase started, where priming was done twice a day (9:00 A.M. and 2:00 P.M.). In this phase, an adult C57BL/6 mouse was introduced to the home cage of the CD-1 mouse only for 30 s and then replaced by an adolescent (PND24–PND25) C57BL/6J mouse for 5 min. Phase 2 lasted 3–4 d until a subset of CD-1 mice became consistently aggressive toward the adolescent C57BL/6J mice. We recorded the latency and the number of attacks made by CD-1 mice toward adolescents in phase 2. Only CD-1 mice that attacked the C57BL/6J at least 10 times on at least two consecutive days were selected as aggressors for the AcSD procedure. The C57BL/6 adult and adolescent mice used for priming were not used for later experiments.

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