In order to standardize procedures between experiments and experimenters, we used an operational definition of attack during AcSD sessions, defined as follows:

One attack is defined as “when the CD1 mouse bites the C57BL/6J mouse, and the C57BL/6J mouse moves away in response to the bite.”

A bite is defined as “when the CD1 mouse places its teeth on any part of the C57BL/6J mouse’s body.”

Moving away is defined as “when the C57BL/6J mouse moves both of its hind paws from the position they were in before the CD1 engaged it.”

There must be ∼2 s between separate attacks. If more than one bite occurs <2 s apart, count as one attack.

If the CD1 mouse bites more than once in succession without a break (<∼2 s apart), gently separate animals.

If the CD1 mouse bites and does not let go, gently separate animals.

If the C57BL/6J mouse becomes trapped in a corner or is pinned down by the CD1 mouse and cannot move away in response to a bite, gently separate animals with a ruler.

In cases 5–7, count as one attack (up to separation).

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