Synchronized day 1 adult wild-type (N2) or HSF-1 overexpression (EQ140) worms were singled onto 35 mm OP50 plates, shifted to 28 °C HS for 24 h, and then shifted back down to 20 °C for recovery. During the recovery period of up to 7 days, adults were transferred to fresh OP50 plates daily and the eggs on each plate were counted. The same plates were scored again the following day for appearance of larvae (viable offspring).

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