Paralysis plates were made using NGM containing final concentrations of 1 mM aldicarb (Adipogen Corporation, San Diego, CA) or 0.5 mM levamisole (Acros Organics, Thermo Scientific, Waltham, MA). For each paralysis assay, drug plates were freshly seeded with OP50 and dried at RT for 24 h. Then, 25 adult worms that had been exposed to 20 °C (control) or 28 °C (HS) for 24 or 48 h (as described for HS conditions) were picked to each drug plate and placed in a 20 °C incubator. Levamisole sensitivity was scored 2 h after plating and aldicarb sensitivity was scored 8 h after plating. Worms were scored as paralyzed if they did not move after being gently prodded 3x on the head and tail with a platinum wire pick. Worms that ruptured or crawled up the sides of the drug plate were censored. The percentage of worms not paralyzed in each trial was calculated from the total number of non-censored worms for that plate.

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