Synchronized wild-type (N2) worms at the late L4/young adult (YA) stage (~ 58 h after synchronization) were singled onto 35 mm NGM plates seeded with 100 μL OP50 and kept at 20 °C for 12 h to reach egg-laying adulthood. The individuals were then transferred to fresh 35 mm plates and shifted to 28 °C for HS or maintained at 20 °C for control conditions (this represents the 0-h timepoint) and the eggs laid on the first plate were counted. Worms were transferred to fresh, pre-warmed plates every 12 h with minimal exposure (< 5 min) to ambient temperatures.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.