Synchronized wild-type (N2) worms at the late L4/young adult (YA) stage (~ 58 h after synchronization) were singled onto 35 mm NGM plates seeded with 100 μL OP50 and kept at 20 °C for 12 h to reach egg-laying adulthood. The individuals were then transferred to fresh 35 mm plates and shifted to 28 °C for HS or maintained at 20 °C for control conditions (this represents the 0-h timepoint) and the eggs laid on the first plate were counted. Worms were transferred to fresh, pre-warmed plates every 12 h with minimal exposure (< 5 min) to ambient temperatures.

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