Human umbilical cords were obtained from healthy donors who understood the study, met the inclusion and exclusion criteria and provided written informed consent. Before preparing the MSCs, the umbilical cords were initially confirmed to be negative for cytomegalovirus (CMV) antigen, anti-human T lymphotrophic virus (HTLV) I/II antibody, anti-hepatitis A virus (HAV) IgM antibody, hepatitis B virus (HBV) antigen, anti-hepatitis C virus (HCV) antibody, hepatitis D virus (HDV) antigen, anti-hepatitis E virus (HEV) IgM/IgG antibodies, syphilis, anti-HIV-1/2 antibodies, fungi, and bacteria. In addition, we limited the age of the donors from 18 to 35.

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