The mitochondrial membrane potential (MMP) was monitored using DiOC6, as described previously [34]. For each condition, 1 × 106 cells were incubated with 1 mL of DePsipher solution (Trevigen, Gaithersburg, MD, USA), which uses a cationic dye (5,5′6,6′-tetrachloro-1,1′,3,3′tetraethylbenzimidazolylcarbocyanine iodide). After incubation for 20 min at 37 °C in a 5% CO2 incubator, cells were washed with 1 mL of pre-warmed 1X Reaction Buffer with Stabiliser Solution and subsequently analysed using an LSR Fortessa flow cytometer (488 nmargonlaser) and the FACSuite software (BD Biosciences).

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