Male study participants self-collected penile swab samples by wiping a dry cotton swab at least three times around the coronal sulcus and the top of the glans. Each swab was then rinsed in 0.5 mL of cobas® PCR Cell Collection Media (Roche Diagnostics GmBH, Mannheim, Germany). Semen samples were obtained at the same time by masturbation after 3–5 days of sexual abstinence. After liquefaction of the ejaculate at room temperature, at least 0.1 mL of ejaculate was put into 20 mL of cobas® PCR Cell Collection media. Clinician-collected cervical brushes were rinsed in 20 mL of cobas® PCR Cell Collection Media. All samples in cobas® PCR Cell Collection Media were transported and stored at room temperature until testing according to the manufacturer’s recommendations for gynecologic samples.

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