CRC cell lines were obtained from the Cell Resource Center (National Infrastructure of Cell Line Resource, NSTI) and cultured in DMEM or RPMI 1640 medium. All medium were supplemented with 10% fetal bovine serum. Cells were maintained in 5% CO2 at 37 °C in incubators with 100% humidity. Cell line authentications were performed by the provider. The antibodies and reagents for this study are listed in supplementary Table S1.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.