CRC cell lines were obtained from the Cell Resource Center (National Infrastructure of Cell Line Resource, NSTI) and cultured in DMEM or RPMI 1640 medium. All medium were supplemented with 10% fetal bovine serum. Cells were maintained in 5% CO2 at 37 °C in incubators with 100% humidity. Cell line authentications were performed by the provider. The antibodies and reagents for this study are listed in supplementary Table S1.

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