Total RNA extraction from EXs was achieved using TRIzol (Invitrogen, Carlsbad, CA, USA). The HiScript Reverse Transcription Kit and ChamQ SYBR Green qPCR Kit (Vazyme, Nanjing, China) were used to perform reverse transcription and quantify RNAs in accordance with the manufacturer’s instructions. The levels of lncRNA-targeted mRNA and lncRNA expression were established using quantitative real-time PCR (the primer sequences used in quantitative PCR are listed in Additional file 1: Tables S1) using a LightCycle 96 system (Roche, Grand Island, NY, USA). U6 RNA was utilized as a control for the numerical analysis. The quantity of mRNA and lncRNA was normalized to the U6 level. Triplet quantitative measurement for each lncRNA or mRNA was performed. A relative normalized expression of each sample was determined based on the level of sample expression at 11 GW.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.