Total RNA extraction from EXs was achieved using TRIzol (Invitrogen, Carlsbad, CA, USA). The HiScript Reverse Transcription Kit and ChamQ SYBR Green qPCR Kit (Vazyme, Nanjing, China) were used to perform reverse transcription and quantify RNAs in accordance with the manufacturer’s instructions. The levels of lncRNA-targeted mRNA and lncRNA expression were established using quantitative real-time PCR (the primer sequences used in quantitative PCR are listed in Additional file 1: Tables S1) using a LightCycle 96 system (Roche, Grand Island, NY, USA). U6 RNA was utilized as a control for the numerical analysis. The quantity of mRNA and lncRNA was normalized to the U6 level. Triplet quantitative measurement for each lncRNA or mRNA was performed. A relative normalized expression of each sample was determined based on the level of sample expression at 11 GW.

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