An aliquot of the EX suspension (10 μL of vesicles in 90 μL of buffer) was collected after adding 10 μL of 10% glycine, which was then diluted with PBS to a final ratio of 1:200 before lysis with Mammalian Protein Extraction Reagent (Millipore, Billerica, MA, USA). The mean concentration (particles/mL) and diameter (nm) of EXs were calculated using NTA 3.1 nanoparticle tracking software and the Nanosight NS300 system with a G532-nm laser module (Malvern Instruments, Malvern, UK).

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