miR-665 mimics, miR-665 mimics negative control (Control, scrambled invalid RNA sequence), miR-665 inhibitor and negative control (NC, scrambled invalid RNA sequence) were designed and synthesized by GenePharma Corporation (Shanghai, China). After culturing NCI-H226/SK-MES-1 cells for 24 h in plates, the RNA oligonucleotides were transiently transfected into these cells with Lipofectamine TM-2000 (Invitrogen, Carlsbad, CA, USA), respectively. Small interfering RNAs (siRNAs) were designed for silencing TRIM8 gene expression. TRIM8 siRNA and negative control siRNA (NC-siRNA, scrambled invalid siRNA sequence) were synthesized by GenePharma Corporation (Shanghai, China). These siRNAs were transfected into NCI-H226/SK-MES-1 cells with Lipofectamine TM-2000 according to the manufacturer's protocol and diluted to 60 nM for the experiments.

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