Human LUSC cell lines NCI-H226, NCI-H1703, SK-MES-1 and NCI-H520, and normal human lung epithelial cells BEAS-2B were purchased from the Cell Bank (Shanghai Genechem Co., Ltd, China). All cell lines have been verified and haven’t been infected by other microbe. These cells were cultivated in RPMI-1640 (Gibco BRL, Grand Island, NY, USA), including 20 μg penicillin/mL, 20 μg streptomycin/mL, and 10% (v/v) fetal bovine serum (Gibco BRL, Grand Island, NY, USA) at 37 °C in the humidified incubator comprising 5% carbon dioxide.

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