Seeds of the safflower cultivar (Chuan Hong No1) were collected in 2017 from the Wenjiang experimental field (black loam, sown in September and harvested in May, the permission to collect plant samples has been obtained), College of Agronomy, Sichuan Agriculture University, China, It had been grown under the same growing conditions for a continuous five years. The seed developmental process from flowering until maturation was observed and the seeds were collected at intervals of two days, including a range of 28 d, and the oil content was detected. Seeds from the four development stages (10, 14, 18 and 22 DAF) were collected used for the transcriptome, and three biological duplications were conducted for each development stage. The collected seeds were stored at − 80 °C.

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