In our comparison, we adapted the Charité protocol, which was published on 17 January 2020 (Christian Drosten et al., 2020). We used the E_Sarbeco_F1 and E_Sarbeco_R2 primers with the E_Sarbeco_P1 probe for the detection of the E gene, and the RdRP_SARSr-F2 and RdRP_SARSr-R1 primers with the RdRP_SARSr-P2 probe for detection of the RdRp gene. All primers and probes were used at the concentrations recommended by the protocol. For the PCR reactions, we used the GoTaq® Probe 1-Step RT-qPCR System (Promega Corporation, 2800 Woods Hollow Road, Madison, U.S.).

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.