In our comparison, we adapted the Charité protocol, which was published on 17 January 2020 (Christian Drosten et al., 2020). We used the E_Sarbeco_F1 and E_Sarbeco_R2 primers with the E_Sarbeco_P1 probe for the detection of the E gene, and the RdRP_SARSr-F2 and RdRP_SARSr-R1 primers with the RdRP_SARSr-P2 probe for detection of the RdRp gene. All primers and probes were used at the concentrations recommended by the protocol. For the PCR reactions, we used the GoTaq® Probe 1-Step RT-qPCR System (Promega Corporation, 2800 Woods Hollow Road, Madison, U.S.).

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