Main clinical features of lung cancer patients from San Luigi Gonzaga Hospital (a total 57 samples used for IHC) are summarized in Table S3. Analysis of tumor samples was performed following informed consent and with obscured identity (#232/INT). Human lung cancer cell lines A549, H1299, H460 and H522, as well as non-cancer lines HEK 293 T and BEAS (a kind gift from Prof. R. Taulli and Prof.sa M. Brancaccio) were cultured in cultured in RPMI 1640 medium supplemented with 10% FBS 1% glutamine and 1% penicillin/streptomycin. All cell lines were maintained in a humidified incubator with 5% CO2 at 37 °C. Each cell line identity was verified by Short Tandem Repeat (STR) DNA profiling.

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