All of the participants were fasting overnight, and 5 mL of peripheral venous blood was collected in the morning. The blood was then allowed to clot in a water batch of 37 °C for 30 min, and followed by centrifugation at 3000 rpm for 15 min. Then the serum supernatant was taken, immediately freezed in liquid nitrogen, and stored at − 80 °C until further analyses [18].

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