Female euthymic (nu+ /nu+) mice were purchased from Shanghai Silaike Experimental Animal Company (license no. SCXK, Shanghai 2010 0002; Shanghai, China). At age 4–5 weeks, the mice were randomized and injected with cells [A549 (vector), A549 (ALPL), HCC827 (vector), and HCC827 (ALPL)] via the lateral tail vein (3 × 106 cells in 100 μL PBS/mouse). Detailed experimental methods have been described previously [27].

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