Tube formation assay was conducted on HUVECs under the treatment of different LUAD cancer cell culture medium. HUVECs (2.0 × 104 cells) were cultured in the presence of the conditioned medium in 96-well plates coated with Matrigel (50 μL/well, BD Biosciences). After 4 h incubation at 37 °C, tube formation was visualized under light microscope. Capillary length was calculated with Image J software. This assay was carried out in triplicate.

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