Saliva was collected using passive drool SalivaBio collection tubes (Salimetrics) and stored at -30 °C. Saliva was then centrifuged, aliquoted, and stored at -80 °C. Cortisol concentrations in saliva samples was determined using Salimetrics High Sensitivity Salivary Cortisol enzyme-linked immunosorbent assay (ELISA) kits. Blood was collected using standard venipuncture procedures with BD vacutainer tubes, cell preparation tubes with sodium citrate for Peripheral Blood Mononuclear Cell (PBMC) isolation, and serum vacutainer tubes with clot activator. A total of 25 mL of blood was drawn during the entire study by a trained phlebotomist. PBMCs were aliquoted and stored in liquid nitrogen and serum was isolated and stored at -80 °C. Interleukin 6 (IL-6) and C-reactive protein (CRP) levels in the serum samples were assayed using MesoScale Discovery V-PLEX assay kits using the MESO QuickPlex SQ 120. Ribonucleic acid (RNA) was isolated from PBMCs using Qiagen RNeasy Micro kits and a complimentary deoxyribonucleic acid (cDNA) bank was created using Omniscript kits. Gene expression analyses were completed by quantitative real-time polymerase chain reaction (qRT-PCR) using the QuantStudio 12 K Flex Real-Time PCR System for a specific gene of interest (i.e., FKBP5) and one control gene (i.e., GAPDH). Saliva samples were collected between 9:30–18:00, whereas blood samples were collected prior to 15:00.

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