Cell migration and invasion were evaluated using kits from BD Falcon. The assay was performed according to the manufacturer’s instructions, as described previously [27, 31]. Briefly, cells were seeded in the inserts in 400 μL serum-free medium. The inserts were placed in wells containing 700 μL medium with 10% FBS and incubated for 24 h. Then, cells on the upper surface of the filters were removed with a cotton swab. The inserts were fixed in methanol and stained with Giemsa, imaged with a microscope, and then the cells were counted. The data shown are representative of three independent experiments [32].

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