Fast SYBR Green Master Mix (4385614; Applied Biosystems/ThermoFisher Scientific) was used for real-time PCR with the 7900HT Fast Real-Time PCR system (Applied Biosystems) [29]. The primers used in this study were as follows: human ALPL (forward: 5′- AC CGA GAT ACA AGC ACT CCC ACT-3′, reverse: 5′- CC GTC ACG TTG TTC CTG TTC AG 3′), human RhoA (forward: 5′-ACT ATG TGG CAG ATA TCG AGG TGG A 3′, reverse: 5′- TA TCA GGG CTG TCG ATG GAA AAA C-3′), human c-Myc (forward: 5′-GGA GGA ACA AGA AGA TGA GGA AGA AA -3′, reverse: 5′-TGA GGA CCA GTG GGC TGT GAG G-3′), and human GAPDH (forward: 5′-GAC TCA TGA CCA CAG TCC ATG C-3′, reverse: 5′-CAG GTC AGG TCC ACC ACT GA-3′).

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.