Fast SYBR Green Master Mix (4385614; Applied Biosystems/ThermoFisher Scientific) was used for real-time PCR with the 7900HT Fast Real-Time PCR system (Applied Biosystems) [29]. The primers used in this study were as follows: human ALPL (forward: 5′- AC CGA GAT ACA AGC ACT CCC ACT-3′, reverse: 5′- CC GTC ACG TTG TTC CTG TTC AG 3′), human RhoA (forward: 5′-ACT ATG TGG CAG ATA TCG AGG TGG A 3′, reverse: 5′- TA TCA GGG CTG TCG ATG GAA AAA C-3′), human c-Myc (forward: 5′-GGA GGA ACA AGA AGA TGA GGA AGA AA -3′, reverse: 5′-TGA GGA CCA GTG GGC TGT GAG G-3′), and human GAPDH (forward: 5′-GAC TCA TGA CCA CAG TCC ATG C-3′, reverse: 5′-CAG GTC AGG TCC ACC ACT GA-3′).

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