Normal bronchus epithelial cell line Beas-2B and lung cancer cell lines A549, H1975, HCC827, H1299, and SK-MES-1 were purchased from the American Type Culture Collection (ATCC; Manassas, VA, USA). All cells were subjected to DNA analysis and authenticated before use in these studies [26]. A549 and SK-MES-1 cells were cultured in F12K (21127-022; Gibco/ThermoFisher Scientific, Waltham, MA, USA) supplemented with 10% fetal bovine serum (FBS) (10437-028; Gibco). H1975 cells were cultured in a 1:1 mixture of DMEM/Ham’s F12 medium (10565-018; Gibco) supplemented with 5% FBS. HCC827 and H1299 cells were cultured in RPMI 1640 (11875-093; Gibco) supplemented with 10% FBS, and Beas-2B cells were cultured in DMEM (11995-065; Gibco) supplemented with 10% FBS. Cell transfections were performed using PolyJet DNA In Vitro Transfection Reagent (SignaGen Laboratories, Rockville, MD, USA) according to the manufacturer's instructions. For stable cell line selection, cells were treated with G418 (500–1000 μg/mL) or puromycin (0.2–0.3 μg/mL) depending on the antibiotic resistance plasmid used. Cells surviving the antibiotic selection were pooled as stable mass transfectants [27].

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